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ods for risk assessment and classification of chemicals. When establishing new test methods, it is imperative to implement the 3R principle where possible. Ideally such tests should be animal-free and should rely on human-relevant biological systems (Beken et al., 2016;Knudsen et al., 2019).The comet assay is the gold standard in many laboratories for genotoxicity measurements. With its high sensitivity and broad scope of application, it is an attractive technique for analyzing DNA damage induction and repair in a broad variety of cells. Nevertheless, a problematic issue is the lack of inter-laboratory comparability (Ersson et al., 2013). Work on standardization of the comet assay protocol has decreased the inter-experimental and inter-laboratory variability of the assay (Cassano et al., 2020), but also shown its drawbacks (Brunborg et al., 2018;Enciso et al., 2018). The CometChip ® platform provides a high-throughput DNA damage analysis in a 96-well format, but no automated version has been described so far (Sykora et al., 2018). Additionally,