Sleep quality is related to daily performance and stress. The working conditions imposed by the coronavirus disease 2019 (COVID-19) pandemic have impacted individuals and families. To analyze the relationship between sleep quality, daily activities, and stress of workers during the pandemic, a study of activity diaries of 113 workers in Havana, Cuba, in August 2020, with data collected by telephone, was carried out. Descriptive and inferential statistics, regression, and social microsequence analysis were used to study the data. Women slept 8.64 hours a day, dedicated 5.30 hours to leisure, 3.53 hours to work, and 3.40 hours to household chores. Men slept 8.33 hours a day, dedicated 6.64 hours to leisure, 4.12 hours to work, 2.32 hours to personal needs, and 1.99 hours to household chores. There were statistically significant differences by sex in terms of role changes, time spent on leisure activities and on household chores, and the number of roles experienced. Women had a positive and statistically significant relationship between the level of rest and time spent sleeping, while men had a negative and statistically significant relationship between the level of rest and hours in household chores. Men slept and rested more than women. The findings corroborate different behaviors by sex, according to the activities and roles they perform in different environments, and their influence on sleep quality and stress.