Multiple sclerosis (MS) is a demyelinating ailment in which the loss of myelin destructs conduction along the affected axons typically resulting in the conduction block. The motive of this protocol is to carry out the neuroprotective activity of Artemisia vulgaris (Mugwort) by induced demyelination of ethidium Bromide in wistar rats. The leaf methanolic extract was treated at 100mg/kg and 200mg/kg weight administered through oral route and continued for 28 days in demyelinated rats. Demyelination was affected by administering intracranial injection of toxin (ethidium Bromide) at the dose of 1µg/0.03ml of PBS per kg body weight. The potency of the extract was analysed in the terms of their behavioural study on the first, second and fourth week. The animals were immolate after 28 days and subjected to histopathological assessment. The unearthing from behavioural histopathological and biochemical studies evince that the methanol extract of Artemisia vulgaris have potential protective effect on the ethidium bromide induced demyelinated rats.