BACKGROUND The aim of the study is to determine the normal development of pituitary volume in Indian adolescent population by means of direct measurements made on thin-section 3D MR acquisitions. MATERIALS AND METHODS 200 children and adolescents in the age group of 10 to 19 years with no abnormal findings on routine MR studies were subjected to the study. The volume of pituitary gland was measured in these subjects using 3D MR sequences with a section thickness of 1 mm. The simplified ellipsoid formula-length x height x width/2 was used for calculation of the volume of the pituitary. RESULTS The mean pituitary volume was 0.42±0.16 cc in females and 0.40±0.16 cc in males. There was a gradual increase in pituitary height with age. Females had a slightly larger pituitary gland, but the difference was not statistically significant. CONCLUSION In this study, we used 3D MR volumetry for the calculation of pituitary volume in normal Indian children and adolescents. Using this technique, we found that females had a slightly larger pituitary volume compared to males.