In the present study, we investigate the serum cholesterol level and the liver enzymes (alkaline phosphatase, alanine transaminase, and aspartate aminotransferase) in mice. Lindane (δ- isomer), 100mg/kg body weight, injected subcutaneously for 30 days. Our present study found that after 30 days of lindane administration, after sacrificing the mice, a significant increase in serum cholesterol levels observed. Alkaline phosphatase levels significantly decreased, whereas the alanine transaminase and aspartate aminotransferase levels significantly increased.