Presence of metals in air, water, soil, and food is due to natural as well as anthropogenic activities (Beiger and Jernelov, 1986). Some heavy metals like Cu, Fe and Zn are essential to maintain proper metabolic activity in living organisms; others like Pb and Cd are non-essential and have no biological role (Qin et al., 2009). However, at high concentrations, even essential metals also cause toxicity to living organisms (Li et al., 2005).The metals present in the milk are a result of exposure of the animal to certain factors like eating grass contaminat- Research ArticleAbstract | Presence of heavy metals in milk, particularly the non-essential ones poses a great risk to both animal and human health. Industrialization, construction, vehicle dust, heavy use of pesticides are some of the major pathways through which these metals gain access into the food chain, and eventually into the milk. The present study was conducted to determine the concentration of heavy metals in buffalo milk, owing to its high consumption in the country. Fresh, raw buffalo's milk samples, total 100 in number were collected from different dairy farms in Mumbai suburbs. After collection, the sample were immediately kept in the freezer (-20˚C) and later processed by acid digestion method using 65% HNO3 and HClO4. Using Atomic Emission Spectrophotometry (ICP-AES), concentration of As (Arsenic), Hg (Mercury), Pb (Lead) and Cd (Cadmium) were evaluated. The results showed high levels of lead and mercury in Borivali (p<0.05)and Bhiwandi (p<0.01) region respectively. The positive values confirm presence of these toxic metals in the surrounding environment. However, no significant levels (p>0.05) of these metals were found in the water which is considered as the most common source of exposure. Also, no correlation was found between levels of heavy metals and its effect on milk yield. These results demonstrated that milk produced in the vicinity of Mumbai is contaminated with heavy metals lead and mercury; moreover environmental pollution could be a potential source of heavy metal contamination in raw milk.