This study aimed to determine the seroprevalence of Toxoplasma gondii, rubella, and cytomegalovirus among 71 pregnant women with age range from 19 to 45, who had mischarge as adverse reproductive outcome in current pregnancy in Albyda city, Libya. Peripheral blood samples were obtained from women how had mischarge recently for laboratory tests, IgM and IgG antibodies against T. gondii, rubella, and CMV agents were detected by ELISA and results were expressed qualitatively as positive and negative. Among the tested sample Highest percentage was for IgG Rubella infection (98.59 %) followed by IgG CMV (96.36%), and IgG toxoplasma (39.39%). This study suggested that between the Rubella, Toxoplasma gondii, and cytomegalovirus infections during pregnancy cause the foetal loss.