HighlightsWe showed the direct interaction of AhR and HSP90 using purified protein.The ligand 17-DMAG induces a dissociation of HSP90 from AhR.The AhR–HSP90 complex is not affected by the timing of β-naphthoflavone binding to AhR.The AhR–HSP90 complex was translocated to the nucleus after treatment with β-naphthoflavone.
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