No abstract

The 16 cases of Clonorchis sinensis infection were divided into 6 groups, according to the method of administration of 1, 4-Bis-trichlormethyl-benzol. The number of egg-out and clinical manifestation of pre.and post drug administration were observed. 1. In all groups, the number of ova transiently increased at the early stage of treatment and followed rather abrupt decrease reaching to E.P.G. zero by Stoll's method on 4 weeks after the medication, except group 6 which showed a few ova by M.G.L. method. 2. Subjective symptoms: fatigability, disappetite and other general complaints by the drug administration were markedly improved in all cases. 3. Blood counts and bleeding time were within normal value before and after the treatment. 4. Liver function test was normal during pre-and post-treatment except case No. 9. Serum alkaline phosphatase of No.9 was high level lbefore treatment, but recovered to noraml level 3 weeks later of the drug administrations. 5. SGOT and SGPT in 10 cases examined showed normal value. 6. The level of serum protein was not changed during observation period. 7. Urine albumin was noted in 2 cases before medication, but they also converted to negative within 3 weeks. Serum creatinine were all normal limits. 8. Bradycardia were noted in 2 cases during observed no significant abnormalities of EKG. Serum electrophoresis were also normal limits.

Trichomonas tenax(T. tenax) and Trichomonas hominis (T. hominis) were collected, cultured and sampled for comparative microscopical studies using electron microscope. 1. Both flagellates were oval in shape and surrounded by a distinct outer membrane. Five recurrent flagella and one anterior flagellum had, each, 9 paris of peripheral and 1 pair of central fibrils, Undulating membrane was curved over the recurrent flagella, and bended in the middle at right angles with cell surface. Cytostome, engulfing bacteria, was observed in T. hominis. 2. In the cytoplasm, there were fine dense glycogen particles, and vacuoles containing ingested materials. Dense pigment rods were also observed in both flagellates, but the rods were not distributed around the vacuoles in T. hominis. 3. In T. tenax axostyle appeared as a cup-shaped structure comprising a single row of 41 fibrils, each about 120 a in diameter. It enclosed glycogen particles, and the open side was faced to the nucleus. 4. Endoplasmic reticulum was observed around the nucleus, but it was less developed in T. hominis. 5. Nucleus was ovoid having double nuclear membrane, which was clearly defined in T. hominis. 6. Blepharoplast, parabasal body, Golgi appartus and mitochondrion was not observed in both flagellates.

A complete but abnormal form of Taenia worm was obtained from an 18-year-old male student in Cheju Island, Korea, which has been known as a heavy endemic area of Taenia saginata and few of T. solium. There was an apical hookless eminence in the scolex, longitudinal white line along the middle portion of entire strobila, double genital pores and several lateral buddings from the strobila. It was considered an abnormal form of Taenia species, probably T. saginata.

The efficacy and toxicity of 1, 4-Bis-trichlor-methyl-benzol were examined in the animal infections(rat, rabbit and mouse) of Clonorchis sinensis. 1)The effective dosages to kill the worm in bile ducts were 1 x 150 mg/kg in rat and 5 x 150 mg/kg in rabbit. 2)During 48 to 72 hours after the administration of the drug, the inactive posteriorly swollen worms and variously degenerated worms were observed from liver ducts, gallbladder and intestine of the experimental animals. In gall bladder of rabbits, a plenty of fragments of the worms and clewed mass of eggs were confirmed under microscope. 3)The E.P.G. was not decreased to zero in 3 weeks observation after the administraiton of the drug except rabbit No. 9 which was treated with the dose of 5 x 250 mg/kg. 4)Minimal congestion and mild cloudy swelling of liver were observed in the groups of mice which were given 5 x 150 kg and 3 x 500 mg/kg of the drug respectively. 5)The body weight of experimental animals showed no evidence of changes.