Antibiotic resistance is a major problem and the greatest challenge to both hospitals and in the community. Initially, antibiotic-resistant strains were found to the hospital environment only, but now they can be present everywhere. It was therefore decided to conduct a short-term study on Andrographis paniculata's ability to inhibit the growth of some of the most resistant bacteria in the Doon Valley. Antimicrobial activity of Andrographis paniculate of leaf and steam extract was performed against different MDR isolates. The VITEK2 COMPACT device with a consistent inoculum was used to identify and evaluate the antimicrobial susceptibility of clinical samples obtained in suspected critical patients at Indresh hospitals in Dehradun District. Clinical and laboratory guidelines were followed during the testing process ( Shri Mahent Indresh Hospital). Steam and leaf extract from A. paniculata leaf was tested for antibacterial activities using the disc diffusion method. The present study showing that the leaf extract and steam extract petroleum ether, acetone and ethyle acetate extract are most active against K. pneumoniae, E. coli, Proteus mirabilis, Pseudomonas aeruginosa, Acetinobacter, Pseudomonas sp. , Enterobacterium (MDR strains) and Candia albugin, Aspergillus niger, Fusarium, penicillium (pathogenic fungus.