No abstract

Prevotella intermedia and fusobacterium nucleatum are associated with various forms of periodontal disease. The purpose of the present study was to infect the clinical isolates of these periodontopathic bacteria and to induce a significant loss of alveolar bone in specific pathogen-free (SPF) rats in the absence of ligatures. P. intermedia YKD8 and F. nucleatum YKZ5 were isolated from a prepubertal periodontitis patient, while P. gingivalis MWB13 was from a patient with juvenile periodontitis. At first, SPF Sprague-Dawley rats (70 days of age, male) were infected with A. viscosus Ny1R and subsequently superinfected with P. gingivalis MWB13, P. intermedia YKD8, or F. nucleatum YKZ5, respectively. The control group was infected with A. viscosus Ny1R alone. All rats were killed and periodontal bone levels were assessed morphometrically 135 days after the first infection with A. viscosus. P. intermedia YKD8 was recovered frequently from rats, with serum antibody levels remaining highly elevated throughout the experiment. Significant loss of alveolar bone was found in rats infected with P. intermedia YKD8, the virulence of which was equivalent to that of P. gingivalis MWB13. F. nucleatum YKZ5 also induced alveolar bone loss, but not significantly when compared with rats infected with A. viscosus Ny1R alone.

Y2Ni7, which is pararragnetic at T=0, becomes ferromagnetic when the temperature is raised to ∼7K; then at ∼60K the system again makes a phase transition to paramagnetic state. We show how it is possible to understand such a behavior of an itinerant electron system if we take into account the effect of the electron-phonon interaction.

We present a case of a triple-X syndrome in a Japanese girl who was treated from 5Y9M to 14Y6M of age at the Pedodontic Clinic of Osaka University Dental Hospital. At the first examination, multiple dental caries were identified, and a pulpectomy and extraction of the affected teeth were carried out. There were no congenitally absent teeth, including third molars, though the development of permanent teeth was approximately 2 years behind chronological age. At 14Y6M, a PCR analysis of 10 putative periodontopathic bacteria was performed, and all 10 species were found in saliva samples. At the same time, an oral examination revealed that the gingival tissues were highly inflamed with an extremely large amount of calculus, suggesting a risk for the early onset of periodontitis.