List of abbreviations: PHA, polyhydroxyalkanoate; PHB, polyhydroxybutyrate; DO, dissolved oxygen; DCW, dry cell weight; COD, chemical oxygen demand; VFA, volatile fatty acid; D-COD, dissolved chemical oxygen demand; GC, gas chromatography; PHB-PHV, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) 2 ABSTRACT We investigated the effects of different feeding regimens (1-pulse, stepwise, and continuous) of fermented food-waste liquid on polyhydroxybutyrate (PHB) production.The fermentation liquid was filtered with a membrane filter (pore size, 0.45 μm) to remove anaerobic microorganisms and solids and used as a carbon source for Cupriavidus necator. One-pulse feeding yielded the highest cell concentration of C.necator. However, the PHB concentration was higher in the stepwise-and continuous-feeding regimens. Therefore, the continuous-feeding regimen was used for continuous PHB production. PHB could be produced over 259 h (8 draw-fill cycles) with a maximal PHB content of 87%, but the PHB concentration and content decreased with an increase in the operation time.