The Zingiberaceae family includes ginger (Zingiber officinale), turmeric (Curcuma longa Linn), galangal (Alpinia galanga), and Kaempferia galanga L contains many compounds, including gingerol, curcumin, and flavonoid, which have natural antioxidant properties. The purpose of this study was to determine the antioxidant activity of extract combination of juice from ginger, rhizome, turmeric, galangal, and Kaempferia galanga L using the DPPH (1,1-Diphenyl-2-Picrylhydrazyl) method and calculate the IC50 value. The combination of ginger rhizome, turmeric, galangal, and Kaempferia galanga L is taken with water or juice using a juicer and then filtered with an abatis cloth. The antioxidant activity test was carried out using a UV-Vis spectrophotometer at a wavelength of 500 nm at 30 minutes. The results showed that the combined extract juice ginger rhizome, turmeric, galangal, and Kaempferia galanga L extracts had antioxidant activity. In formula I, the IC50 value was 23.5 ppm (very strong), formula II IC50 value was 171 ppm (moderate), and formula III IC50 value was 552 ppm (very weak). The conclusion from this research, domination concentration of ginger in combination with rizhoma increases antioxidant activity.