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Resonance Raman spectra of the single-copper blue proteins azurin (from the bacteria Pseudomonas putida, Pseudomonas aeruginosa, Iwasaki sp., Bortadella pertussis, Bortadella bronchiseptica, and Alcaligenes faecalis), plastocyanin (from French bean and spinach), and stellacyanin (from the Japanese and Chinese lacquer trees Rhus vernicifera) and the multicopper oxidases lacease (from the fungus Polyporus versicolor and the Japanese and Chinese lacquer trees), ascorbate oxidase (from zucchini squash), and ceruloplasmin (from human blood serum) are reported. Cryoresonance Raman observations (10-77 K) are reported for selected azurins, stellacyanin, the plastocyanins, and the laceases. Isotope studies employing 63Cu/65Cu and H/D substitution are reported for selected azurins and stellacyanin, allowing identification of modes having significant

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The National Bureau of Standards is a principal focal point in the Fvderal Government for assuring maximum application of Iti. physical and enpinveriina sciences it) the ad~ziiienient oif technology ill iodustr% anid commerivrce. Its responsibilities include development and mainteniaic oif the niational simolar(Is idf measurement, and the provisions of means for making measuremnrirts conskistet %ith tbose slannda rds; determination of t~aical constants arid properties of materials: ditviclopirnt it(o methiods for te-ting materials, rncchari. s, and soructures. and making such tests it-; may be riccessarv, parlicti. larlv for gove-rnrnent agencies; cooperation in (lie establishment of standard piactices for incorpora. lion in codes and specifications; advisory servire to} government ag~encies on scientific tod itechinial prolemsn: inivenrtion and development of devices to serve special needs of fay Laboratory Abtmophy-irs at the Vniver-ity ,I Col.,ratr locaird at ltoulder, h.. 1