The seropositivity of T. gondii has been found to be so large that it should not be ignored. It has come to the conclusion that the events in the risk group should be taken into consideration for this parasite, and awareness should be established. In recent years, this awareness has been observed, especially in neurology clinics.

Toxoplasma gondii is the causative protozoan of toxoplasmosis which has a worldwide distribution among humans and warm-blooded animals. The diagnosis generally depends on serologic tests but the persistently high Immunoglobulin M (IgM) or low IgG avidity complicate diagnosis. It is essential to identify acute-stage-specific antigen to use in a single test for the definitive diagnosis of the acute disease, especially in pregnant women and immunocompromised individuals. In this study, we investigated the recognition of proteins of lysate antigen (LA) and excretory-secretory antigen (ESA) fractions of the virulent RH strain of T. gondii by the sera of patients who had recent or past infections and the sera of experimentally infected mice. As a result, no specific bands for discrimination of recent infection from the past one could be detected in our study but the results confirmed that proteins of approximately 20, 30 and 40 kDa were among the major targets for IgG responses during acute and chronic infection with T. gondii in humans.

Toxoplasma Gondii is a zoonotic parasite showing intracellular localization. Although it doesn't select a specific region in the brain tissue, the cyst forms occurring in the chronic phase may cause behavioral changes when it is located in the brain regions related to behavior. The aim of this study is to investigate whether there is a relationship between neurodegenerative and neuropsychiatric diseases caused by antibodies transmitted to the first generation of T.Gondii infection by behavioral tests. In this study, using a total of 24 female and 6 male rats. Behavioral tests have been applied to F1 generation groups female and male. In this study, female rats with chronic T.Gondii infection have been found to have increased anxiety in open field in the first generation male offsprings and decreased locomotor activity. Increased mobility in male offsprings has been observed in the elevated plus maze test. In female offspring, it has reduced anxiety and depression.

ÖZETAmaç: Toxoplasma gondii dünyada yaygın olarak görülen ve toksoplazmoza yol açan protozoon bir parazittir. Zorunlu hücre içi yerleşim göstermekte ve insanlar dahil tüm sıcak kanlıları enfekte etmektedir. Özellikle immunkompromize olanlarda ve konjenital bulaş sonucunda ciddi klinik tablolara yol açabilmektedir. Hayvanlarda meydana getirdiği düşüklerle de hayvancılığın yaygın olduğu ülkelerde ekonomik kayıplara yol açmaktadır. Tanısı direkt veya dolaylı olarak yapılabilmektedir. Dolaylı tanıda, referans test olan Sabin-Feldman boya testi, ELISA ve immun floresan antikor testi (IFA) gibi serolojik testlerden faydalanılmaktadır. Serolojik testlerde saptanan antikorlar, sentezlenmelerine yol açan antijen yapısıyla ilişkilidir, bu yüzden farklı suşlardaki protein yapılarının incelenmesi önem taşımaktadır. Bu çalışmada T. gondii'nin RH, Ankara ve TS-4 suşları kullanılmış ve protein yapılarındaki farklılıklar incelenmiştir. Yöntemler: Virulan RH ve Ankara suşları fare peritonuna inoküle edilerek, TS-4 suşu ise Vero hücre kültüründe üretilmiştir. İnokülasyondan üç gün sonra, takizoitler periton yıkanarak toplanmış ve parçalanmıştır. Lizatlar yoğunlaştırmak amacıyla liyofilize edildikten sonra protein içerikleri saptanmıştır. Suşların protein yapıları SDS-PAGE ile ayrılmıştır. Yürütülen lizatlar polikromatik gümüş boyası ile boyanmış ve ortaya çıkan bantlar moleküler ağırlık standardı ile karşılaştırılmıştır. Bulgular: Karşılaştırma sonucunda, Ankara ve RH suşunda 60-70 kDa arasında ve 15 kDa seviyesinde yoğunlaşma gözlenirken, TS-4 suşunda 60 ve 115 kDa ağırlığında olanlar en belirgindi. Sonuç: RH ve Ankara suşlarının aynı protein bantlarını içerdiği, TS-4 suşunun daha farklı ve diğer iki virulan suşa oranla daha az protein bantına sahip olduğu anlaşılmıştır. (Turkiye Parazitol Derg 2011; 35: 133-6) Anahtar Sözcükler: Toxoplasma gondii, RH, TS4, Ankara suşu, antijen Geliş Tarihi: 01.12.2010Kabul Tarihi: 21.07.2011ABSTRACT Objective: Toxoplasma gondii the causative agent of toxoplasmosis, is a worldwide intracellular protozoan parasite that infects all warm blooded animals including humans. It can be devastating to immunocompromised humans and congenital transmission may result in severe clinical spectrum. It causes economic losses due to abortus in animals. Toxoplasmosis diagnosis depends on direct and indirect methods. Besides the Sabin-Feldman test, which is accepted to be the reference test, serologic tests such as ELISA and immunofluorescence antibody tests are means of indirect diagnosis. As detected antibodies in serologic tests are correlated with antigens that cause their synthesis, it is important to know different proteins of different strains. In this study RH, Ankara and TS-4 strains were used and differences between their proteins were examined. Methods: RH and Ankara strains were inoculated into the peritoneal cavity of mice. TS-4 strain was produced in Vero cell culture. Tachyzoites collected by peritoneal wash were lysed and lyophilised. This was run on SDS-PAGE gel and protein bands were compared ...