The aim of this study is to synthesize, characterize and screen some new 1-(4-((2-(4-substitutedphenyl)hydrazono)methyl)phenyl)-1H-1,2,4-triazole derivatives for their antimycobacterial activities. Materials and Methods: The target compounds (2a-h) were gained by condensation of 4-(1H-1,2,4-triazol-1-yl)benzaldehyde with appropriate phenylhydrazines. Their structures were elucidated by IR, 1 H-NMR and mass spectrometry. The antimycobacterial activities of the compounds were determined in vitro against M. tuberculosis H37Rv. Results: The biological assay results showed that the methylsulfonyl substituted derivative 2f displayed the highest antimycobacterial activity in this series. Conclusion: Although the methylsulfonyl substituted derivative exhibited significant antimycobacterial activity, none of the synthesized compounds was found as effective as isoniazid, rifampin, ethambutol and ciprofloxazin against M. tuberculosis.