Multichannel pipettes are used widely to accelerate research and testing in life sciences laboratories and within the biomedical industry. Commercial multichannel pipettes cost several hundred to thousand U.S. dollars and are not accessible in many areas. This study utilizes an open source and digital distributed manufacturing model to design a USD$24 4-channel 200 micro-liter pipette. The design uses widely-available interchangeable off-the-shelf parts and custom components, which can be fabricated with a low-cost fused-filament RepRap-class desktop 3D printer. The manuscript describes the design, construction, and validation of an ergonomic open-source multichannel pipette, as well as the evaluation of pipetting parameters and quality control. The open source multichannel pipette is found to be compliant with ISO 8655 standards for precision and accuracy. The device has separate syringes that operate individual tips, which prevents cross contamination of specimens because there is no direct contact of different liquids with each other and no mixing with actuating air. Each syringe is easily replaced for different experiments and the source CAD files are provided to enable others to build variants or custom configurations of the open source multichannel pipette. METADATA OVERVIEWMain design files: https://osf.io/9tn6e/ Target group: chemistry, biochemistry, biology, biomedical, medical, and associated disciplines Skills required: desktop 3D printing -easy, mechanical assembly -easy Replication: https://www.appropedia.org/Open_Source_3-D_Printed_ISO_8655_Compliant_ Multichannel_Pipette for comments and feedback.See section "Build Details" for more detail.