Previous models of disuse have invariably used surgical methods require repetitive plaster casts applications. A method of disuse atrophy that does not require repetitive application is described. A modified plastic pipette tubing was applied to one hindlimb from thigh to foot resulting in immobilization of knee in extension and ankle in plantar flexion position. This method resulted in loss of soleus muscle mass to 11, 22, 39, and 45% at 3, 7, 14 and 21 days, respectively, in association with a significant decrease of tibialis twitch (25%) and tetanic tensions (26%) at 21 days, compared to contralateral side and/or sham immobilized controls. Immunohistochemical analysis of soleus using fluorescent α-bungarotoxin revealed a significant increase in the number of synapses per unit area (818+31 vs 433+16/mm2) and increase in muscle fibers per unit area (117 vs 83/mm2) most likely related to atrophy of muscle fibers bringing synapses closer. A three fold increase in alpha7 acetylcholine receptor (α7AChR) protein expression along with increased expression of α1AChR subunit on immobilized vs contralateral side was observed. The physiology and pharmacology of the novel finding of upregulation of α7AChRs with disuse requires further study.