Background: HS1 is a cortactin homolog, however its role in neutrophil chemotaxis is not known. Results: HS1 interacts with Arp2/3, regulates Vav1 and Rac signaling, and is necessary for efficient neutrophil chemotaxis. Conclusion: HS1 tyrosine phosphorylation is critical for its interaction with Arp2/3 and efficient neutrophil chemotaxis. Significance: This work increases our understanding of how actin regulatory proteins mediate efficient cell migration.
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