The productivity of rice fields in Indonesia is decreasing and production per unit area is also decreasing due to the shifting of functions from agricultural land to non-agricultural land. Excessive land continuous use of chemical fertilizers can damage the soil so the solution offered is the use of biological fertilizers, namely by utilizing the use of microorganisms from nature. This study aims to determine the effectiveness of bacteria G3 and G4c that have been obtained by previous researchers against root weight, root length, root width, root volume, root surface area in rice Var. Ciherang and red rice Var. Barak Cenana. Making NFb media as a selection medium then propagated on TSA media and a confirmation test is carried out. The results showed treatment with G3 (Beijerinckia fluminensis) on rice Var. Ciherang had the highest average root weight, average root length, root width, root volume and root surface area compared to the control and G4c (Rhizobium pusense) treatment. Control treatment without giving G3 (Beijerinckia fluminensis) and G4c (Rhizobium pusense) on red rice Var. Barak Cenana had the highest average root weight, average root length, root width, root volume and root surface area compared to G3 (Beijerinckia fluminensis) and G4c (Rhizobium pusense).