Interests in intracellular peptide delivery have continued to grow, significantly fueled by the importance of peptides and their mimetics in modern cell biology and pharmaceutical industry. However, efficient intracellular delivery of membrane-impermeable peptides of different polarities remains a challenging task. In this study, we develop a general and robust strategy for intracellular peptide delivery by using a boronic acid-rich dendrimer. The designed material is capable of transporting peptides with different polarities and charge properties into the cytosol of various cell lines without inducing additional cytotoxicity. The transduction efficacy and proteolytic stability of cargo peptides delivered by the boronic acid-rich dendrimer are much superior to peptides conjugated with cell penetrant peptides such as octaarginine. In addition, the bioactivities of pro-apoptotic peptides are maintained after intracellular delivery. This study provides a versatile and robust platform for the intracellular delivery of membrane-impermeable peptides.

Abstract

Digital economy, as an emerging economic form, opens a new journey for China's economic and social development, and promotes the shift of the distribution space of human resource management in the public sector, the change of objects of action, the upgrade of information management, and the transformation and development of human demand. However, as far as human resource management in the public sector is concerned under the current digital economy, there are still some urgent problems, such as information security cannot be effectively guaranteed, public officials cannot be properly placed, and management system has not been scientifically established. At present, it is an important task for China's public sector to further improve the system construction, ensure information security, strengthen prediction and analysis, optimize personnel placement, learn from foreign experience, and improve the management system.

Abstract

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