The research on environmental biotechnology has been done in aims to study the ability of mixed culture indigenous bacteria to remove cyanide (KCN-) concentration which is determined by environment conditions of temperature and contact time. The research has been done using the artificial pollutants that contains 500 ppm of KCN- which is exposed to the SMSS (Stone Mineral Salt solution) as a growth media for mixed culture bacteria TBS and CTS. The effect of different environment conditions including temperature and contact time were studied on cyanide removal. Gas Chromatography - Mass Spectrophotometer (GC - MS) method is used to analysed the concentrations of KCN-. Initial condition of research operation is determined to be pH 7 and contact time of 48 hours. The removal of 500 ppm KCN- increases to 71% at incubation temperature 35°C and contact time of 36 hours. This research showed that indigenous bacteria TB1 and CS2 can remove 500 ppm KCN- on all temperature variations between (°C) 25 to 45 and the highest removal occur when contact time is 36 hours.