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Background: Spathiphyllum is one of the best plants for indoor gardening as it is a rhizome and undemanding of light care. It is a member of lily family (Araceae). The plant itself should live for 10 years or more if not maltreated. Spthiphyllum cannifolium plants are used in clean air study for their ability to remove formaldehyde, benezyne, and CO 2 from interior air. UV is divided into three wavelength ranges; UV radiation can have damaging effects on DNA and/or physiological processes and play central role in characterization of bioresponses. A large number of studies have been conducted to evaluate the potential consequences of an increase in UV-B radiation on many plants. Results: In this investigation, plants treated with 45-min ultraviolet radiation (UV) recorded the highest values on growth parameter, anatomical structure, survival %, and proline %. Fifteen minutes of UV radiation exposure gave the second highest value of previous parameters. Conversely, exposure of plantlets to moderate dose (30 min of UV radiation exposure) recorded fewer values in previous mentioned parameters. Start codon targeted (SCoT) analyses exhibited 39 DNA bands which appeared by using seven primers. These bands were identified as 15 polymorphic and 24 monomorphic ones with 38.46% polymorphism. Twelve unique bands were identified in the resulted SCoT profile. Conclusion: The results were nearly in an ascending order with increasing of UV-B radiation exposure time. Increasing exposure time to 45 min increased significantly the values of vegetative growth, anatomical parameters, and proline percentage in most cases compared with control and other UV-B radiation treatments. In the contrary, 30 min recorded decreased in previous mentioned treatments. The SCoT (Start codon targeted) marker is helpful for possible distinguishing, identifying, and characterizing of UV treatments.

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The percent work aimed to establish an efficient in vitro propagation and conservation protocol for Brunfelsia pauciflora plant using encapsulation techniques that allow the genetic stability of conserved material. The effect of plant growth regulators types (BAP, Kin, 2ip, and NAA) and concentrations at 0.0, 1.0, and 2.0 mg/l were stud-ied during the establishment phase in comparison with a hormonal-free medium. In vitro proliferated shootlets were encapsulated using various levels of sodium alginate at 1, 2, and 3% (W/V) and calcium chloride at 2.5,5 and 10 %. The regrowth ability of encapsulated explants was evaluated after conservation for 3, 5, and 7 weeks and compared with non-encapsulated buds. The findings of the current study proved that during the establish-ment phase, applying BAP at 2.0 mg/l gave the best results during the three successive subcultures. The best treatment 2ip (2.0mg/l) + BAP (2.0mg/l) + NAA (2.0mg/l) that was considered ideal propagation protocol for shooting and rooting of Brunfelsia. Successful plant recovery from encapsulated explants using low concentra-tions of sodium alginate (1%) and calcium chloride at 2.5% proved that the explants could to survive at the high-est percent (100%) during conservation periods 3, 5, and 7weeks. Increasing calcium chloride to 5% with the same concentration of sodium alginate (1%) caused a gradually reduced percent of survival to 53.33% with an increasing conservations period (from 3 to 7weeks). It is the first paper on the application of the syndetic seed technique on Brunfelsia for the shortest period of conservation and successive renovation of shootlets whose genetic stabilization was evaluated by analysis of RAPD that proved that the genetic similarity ranged from 56.1 to 80%. These results were confirmed by anatomical sections made in Brunfelsia leaf blade.

The results indicate that the application of NPK fertilizer at the different rates gave a better effect on all vegetative characteristic{plant height (cm), stem diameter (cm), fresh weight of leaves, stems and roots (gm/plant), dry weight of leaves, stems and roots (gm/plant), leaf area, number of roots and root diameter } and chemical constituents ( chlorophyll a,b and carotenoids, mineral contents of N, P and K in the leaves and total sugar contents in the leaves) compared with the control. Also, the results indicated that the application of NPK fertilizer at (3:1:1) was the most effective treatment gave significant increases in all vegetative characteristics and chemical constituents in both seasons.