Background:The diseases that vaccines prevent can be dangerous, or even deadly, especially in infants and young children. Aim: assess the Effect of Nursing Performance on Quality of Care for Infant / Children Vaccination. Design: descriptive research design was used in this study. Setting: The study was carried out in all family medical centers at Giza city. Subject: A purposive sample was used to choose nurses and mothers that equal 135 for everyone. Tools: Interviewing questionnaire divided to two parts as Socio-demographic characteristic, and nurses' knowledge. The second tool: Observational checklist to assess nurses performance and the third tool that divided to two parts; Mothers' satisfaction, and Mothers perception regarding nurses' quality of care Results: This study showed that 52.6% of nurses had poor total level of Knowledge, 91.9% of nurses their total level of Performance was ranged between poor and average and 48,9% of mothers had agree satisfaction about children vaccination. Conclusion: Statistically, significant positive correlations were detected between nurses performance, mothers satisfaction, and mothers perception regarding quality of care about children vaccination. Recommendation: Developing an educational program that would help nurses to improve knowledge and performance about vaccination.