Lake Lebo Taliwang is one of the lakes in the West Nusa Tenggara with high freshwater fish species diversity. However, the species identification of freshwater fish species from Lake Lebo Taliwang using DNA barcoding is very limited. Therefore, the objective of this study was to identify seven samples of freshwater fish species collected from Lake Lebo Taliwang based on COI mitochondrial gene as a DNA barcoding marker and establish library COI sequences of Indonesian freshwater fish. We are using a standard DNA analysis and data obtained from this study was then examined using Nucleotide BLAST and the phylogenetic tree was analyzed using the Neighbour-Joining (NJ) method with Kimura 2 Parameter (K2P) model. The results revealed that among the seven samples of freshwater fishes collected from the Lake Lebo Taliwang, three samples were identified as Anabas testudineus, the other three samples were Barbodes binotatus and one sample was Trichopodus trichopterus. The level of similarity of these freshwater fish samples referred to the database from the GenBank and BOLD was between 98-100%. The NJ tree supports the clade of each species identified in this study. This occurrence indicated that DNA barcoding by using the COI mitochondrial gene was proven to be able to identify the freshwater fish samples accurately.

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Watu Joglo is one of the caves located in the row of the Sewu mountains, Gunung Kidul, Special Regency of Yogyakarta. Watu Joglo has various suitable microhabitats for herpetofauna. So far, the study about herpetofauna’s community structure and distribution has not been carried out in Watu Joglo. This study aimed to study and map the diversity, richness, evenness, and distribution of herpetofauna in Watu Joglo. Herpetofauna’s data sampling was carried out for three days. The study was conducted on 6-8 April 2018. The data of herpetofauna community were analyzed with Shannon-Wiener diversity index, Margalef richness index, and Pielou Evenness Index. The data of herpetofauna distribution were mapped and analyzed with Avenza and ArcGis. Based on the research conducted, we found 11 species of herpetofauna which consisted of five species from Ordo Anura, four species from Subordo Lacertilia, and two species from Subordo Serpentes. Herpetofauna in Watu Joglo was widely distributed around the river. According to diversity analysis, Watu Joglo has moderate diversity index, 1.667. Besides, Watu Joglo has high evenness index which is 0.85 but low richness index which is 2.83.