Objectives: It's known that cigarette smoking affects semen quality. The aim of the present study is to compare the semen of infertile cigarette smokers with infertile non-smokers as well as with healthy men to examine the impact of smoking on the quality of sperm. Methodology: A case-control study was applied in the present study. For the period of 1st January till the 1st April 2011. The semen quality of men with infertility, smokers (n 150) and nonsmokers (n100), were compared with (n 100) healthy- nonsmokers men. Conventional semen analysis was performed and sperm morphology was assessed by transmission electron microscopy (TEM). Smoker patients were classified as mild (1-10 cigarettes/d), moderate (10 -20 cigarettes/day), or heavy smokers (more 20 cigarettes/d).data was analyze through inferential statistics. Results: The study finding showed that sperm parameters quality in smoker men was approximately lower than nonsmoker men and the cigarette smoking and the dose has significant negative effect on sperm parameters. Conclusion: The results of the present study suggest that smoking negatively affects male fertility. Recommendation: Smokers should quit smoking for the sense of responsibility for their future generation as tobacco smoke contains numerous mutagenic substances.