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This study aims to determine how the implementation of Pancasila values in Scouting activities and what are the inhibiting factors and supporting factors for the Implementation of Pancasila values in Scouting activities. The benefits of this study use the theoretical benefits and practical benefits. This type of research is descriptive with a qualitative approach. Data collection techniques and data analysis techniques using interviews, and documentation. The results of this study indicate that: (1) The application of the divine value is evident from the observance of the members of the Boy Scouts in practising the teachings of Religion. The application of human values can be seen from the love between Scout members. Application of the value of unity seems to be carried out activities always prioritize to foster a sense of togetherness. The application of popular values can be seen from the preservation of democracy in scouting activities. Fairness values can be seen from the tolerance level of Scout members, as well as the Scoutmaster who is being fair. (2) Inhibiting factors lack of self-awareness to obey the rules, lack of cooperation in the task, the number of negative behaviours from outside. And Supporting Factors become more independent individuals, raise awareness, train discipline, learn Scout organizations well and train leadership, improve themselves to always apply the values of Pancasila such as diligently worshipping so that hearts and minds become calm.Keywords: Pancasila, the implementation of the values of Pancasila, ScoutingPenelitian ini bertujuan untuk mengetahui bagaimanakah Implementasi nilai-nilai pancasila dalam kegiatan Kepramukaan dan apa faktor penghambat dan faktor pendukung Implementasi nilai-nilai pancasila dalam kegiatan Kepramukaan. Manfaat penelitian ini menggunakan manfaat teoritis dan manfaat praktis. Jenis penelitian yang digunakan adalah deskriptif dengan pendekatan kualitatif. Teknik pengumpulan data dan teknik analisis data menggunakan wawancara, dan dokumentasi. Hasil penelitian ini menunjukkan bahwa: (1) Penerapan nilai ketuhanan tampak terlihat dari ketaatanan aggota pramuka dalam mengamalkan ajaran-ajaran Agama. Penerapan nilai kemanusiaan tampak dari rasa kasih sayang antar anggota Pramuka. Penerapan nilai persatuan tampak kegiatan yang dilakukan selalu mengedepankan untuk memupuk rasa kebersamaan. Penerapan nilai kerakyatan tampak dari terjaganya demokrasi dalam kegiatan kepramukaan. Nilai Keadilan tampak dari tingkat toleransi anggota Pramuka, serta Pembina Pramuka yang bersikap adil. (2) Faktor Penghambat kurangnya kesadaran pada diri sendiri untuk patuh pada peraturan, kurangnya kerjasama dalam tugas, banyaknya perilaku negatif dari luar. Dan Faktor Pendukung menjadi pribadi yang lebih mandiri, meningkatkan kepedulian, melatih kedisiplinan, belajar organisasi Pramuka dengan baik dan melatih kepemimpinan, lebih membenahi diri untuk selalu menerapkan nilai-nilai Pancasila seperti rajin beribadah agar hati dan pikiran menjadi tenang.Kata kunci: Pancasila, implementasi nilai-nilai Pancasila, Kepramukaan.

In the diagnosis of glioblastoma multiform (GBM), there is often a disagreement between the WHO based astrocytoma grading and the patient's clinical outcome. NF-κB is assumed to be a good predictor of clinical outcome of astrocytoma. This study will determine the relationship of NF-κB expression with astrocytoma grade and clinical outcome. This analytical cross sectional study were conducted on 25 astrocytoma samples. Samples were analyzed by anatomical pathologists to determine WHO grading and immunohistochemically stained to analyze the expression of cytplasmic and nuclear NF-κB. The clinical outcome was determined from the patient's survival at the end of therapy. The results showed that majority of astrocytoma patients were in grade I (36%), had positive cytoplasmic NF-κB expression (88%), and negative nuclear NF-κB expression(40%). A lower grade of astrocytoma and better outcomes were found in samples with positive cytoplasmic NF-κB expression or negative eotide NF-κB expression. However, no cytoplasmic or nuclear NF-κB expression relationship were found either with astrocytoma degree (p = 0.543; p = 0.442) as well as clinical outcome of patients (p = 0.472; p = 0.378). Thus, this study shows that NF-κB can not be used as a predictor of prognosis in patients with astrocytoma. Further research is needed to reassess this relationship with more focus on high grade astrocytoma.

:A simple liquid chromatography-tandem mass spectrometry (LC-MS/MS) method for quantifying paraquat(PQ) level was described. Ethyl paraquat (EPQ) was used as an internal standard. The effects of varying the mobile phase composition, flow rate and splitter ratio were investigated. Analytes detection was conducted using a tandem mass spectrometer in multiple reactions monitoring (MRM) modes with the electrospray ionization (ESI) source operated in a positive ion mode. To optimize the MS-MS condition, the effects of varying instrumental parameters such as capillary voltage, collision energy, drying gas and spray chamber temperature as well as nebulizing gas pressure were investigated. Initial tissue sample preparation involved a simple one-step protein precipitation using acetonitrile. Chromatographic separations of PQ and EPQ were successfully performed on an HILIC column (3 µm; 150 × 4.6 mm) with mobile phase 250 mM ammonium formate/ acetonitrile (6:4 v/v). The flow rate was 1 ml/min and reduced to 0.1 ml/min for MS detection using splitter. The MRM transitions (precursor ion/product ion) for quantitation were m/z 186/171 for PQ and m/z 107/185 for EPQ. The optimized condition for MS-MS detection include: drying gas, 375 °C, 15 psi; nebulizing gas pressure, 50 psi.Overall, the method provides a simple and direct analysis for detection of PQ with a total run time of less than 10 minutes and is applicable for quantification of PQ in biological tissue sample with good recovery and precision achieved.