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Two new potentiometric sensors were created for the quantification of bisoprolol fumarate and alverine citrate in bulk pharmaceutical dosage forms and human serum. Bisoprolol and alverine sensors were manufactured by combining potassium tetrakis (p-chlorophenyl) borate ion pairs to serve as electroactive substances, plasticized poly (vinyl chloride) matrix membranes, and o-nitrophenyl octyl ether. They demonstrated high responses over the concentration ranges of 1.0×10−6 to 1.0×10−2 mol L−1 bisoprolol and alverine with close to Nernstian cationic slopes of 52 and 56 mV decade−1, respectively. The detection limits for bisoprolol and alverine were 2.6×10−6 and 1.75×10−6 mol L−1, respectively. For both medications, the response time was instantaneous (2.0 s). The working pH ranges for bisoprolol and alverine were 4.50–8.50 and 2.00–8.80, respectively. For both sensors, the life cycle was long (3 months). The sensors were used in pharmaceutical dosage types for the assay of bisoprolol and alverine, recording average recoveries of 99.40% and 99.98% respectively and were also successfully used for estimating the two drugs in human serum with an average recovery of 99.60% for both drugs. For all multiple staged interfering materials, the reported latest potentiometric sensor methods displayed high selectivity. The current sensor obtained a high percentage recovery and an excellent relative standard deviation compared with those obtained from previously published methods.

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A rapid and accurate reversed phase-UHPLC method is developed for determination of gallic acid, quercetin and kaempferol in herbal drugs found in herbal product purchased from local market. A reversed phase chromatographic analysis is carried out under isocratic conditions using column Phenomenex, Prodigy, ODS3, 5.0 μm, 100 A, 250 × 4.6 mm (USA). The mobile phase is methanol and 0.5 % phosphoric acid (50:50 v/v). The flow rate is 1.3 mL min-1. The injection volume is 20 µL. The detection wavelength (λ max) is 370 nm using a PDA (photodiode array detector). Linearity of the method is established over the concentration ranges of 240-960 µg mL-1 for quercetin with a retention time about 7.8 minutes. Correlation coefficient is greater than 0.99. The recovery level of quercetin is 99.94 %. The described method is quite suitable for routine analysis of herbal drugs by reversed phase-UHPLC Method. The proposed method is used for routine analysis to estimate the selected polyphenols under study in its pure form as well as in some herbal drugs forms.

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