This work used the generally accepted modern methods and programs for the variety study of fruit plants developed at the Research Institute of Horticulture, Viticulture and Winemaking named after Academician Makhmud Mirzaev. The aim of the study was to select the source material of the apple fruit quality introduced in Tashkent region and 35 local varieties of apples, which is valuable for the breeding, and the sources of the target traits based on the analysis of long-term data on the assessment of the biochemical composition of promising varieties of different apples. According to the results, Williams Pride, “Isroil”, Kuban crimson, Esaul’s memory, and Mutsu varieties differed from other varieties with high quality.