Basil leaves and basil seeds have been shown to contain secondary metabolites such as alkaloids, flavonoids, tannins, polyphenols, essential oils that have the potential as antibacterial. Basil seeds are produced from the seeds of the basil flower. The aim of the study was to identify the chemical constituents and compare the antibacterial potential of basil leaves and flowers (Ocimum sanctum L.) against Propionibacterium acnes. Antibacterial testing method with agar diffusion disk using blank paper disc and Nutrien Agar media. Basil leaves and flowers were macerated with ethanol, then prepared as test materials for concentrations of 10, 20 and 40 mg/ml. The results showed that basil leaf extract contains alkaloids, tannins, flavonoids and polyphenols. It was concluded that basil leaves and flowers may act as antibacterial Propionibacterium acnes at concentrations of 10 mg/ml, 20 mg/ml and 40 mg ml. Basil leaves are more potent than basil flowers as an antibacterial Propionibacterium acnes with an optimal concentration of 20 mg/ml, but the amount of chemical compounds in the basil flower extract is higher.