~~ ~Microbial death was studied under starvation conditions. The kinetics of cell death were described by dN/dt = KN"+ l , where N is the number of viable cells at time t, and K and 01 are constants concerning growth or death of bacteria. Death in the decline phase of the culture alsd fitted the above equation, except for irregular oscillations with relatively short periods. This suggested that bacterial death in the decline phase was mainly caused by the same factor(s) as death under starvation conditions.
In the present report, a highly selective tellulite agar medium is described, which has a marked inhibitory effect on growth of many strains of Escherichia, Salmonella, Shigella, Proteus, Bacillus, Staphylococcus, Pseudomonas and most vibrio but allows growth of Vibrio parahaemolyticus and El Tor Vibrio. This medium was named TTGA agar and consists of: gelatin 10g/L, NaCl 30g/L, Na-taurocholate 5g/L, Na2CO3 1g/L, K-tellulite 10mg/L, and agar 15g/L.The laboratory investigations detecting Vibrio parahaemolyticus in feces of food poisoning patients suggested that this medium is adequate for field trials.
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