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Mit Hilfe der PERKOW-Reaktion werden Phosphoenolbrenztraubensaureester und Enolphosphate von Estern dargestellt; besonders leicht laBt sich das Enolderivat des Malonesters (VII) bereiten. Mit Sauren bildet sich aus VII Malonester und unsymmetrisches Pyrophosphat bzw. Acylphosphat ; auf diese Weise werden Verbindungen wie Acetylphosphat, Benzoylphosphat, Tosylphosphat u. a. bereitet. Eigenschaften und IR-Spektren dieser Verbindungen werden diskutiert.CRAMER und GARTNER Jahrg. 91

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Corynebacterium glutamicum is the major host for the industrial production of amino acids and has become one of the best studied model organisms in microbial biotechnology. Rational strain construction has led to an improvement of producer strains and to a variety of novel producer strains with a broad substrate and product spectrum. A key factor for the success of these approaches is detailed knowledge of transcriptional regulation in C. glutamicum. Here, we present a large compendium of 927 manually curated microarray-based transcriptional profiles for wild-type and engineered strains detecting genome-wide expression changes of the 3,047 annotated genes in response to various environmental conditions or in response to genetic modifications. The replicates within the 927 experiments were combined to 304 microarray sets ordered into six categories that were used for differential gene expression analysis. Hierarchical clustering confirmed that no outliers were present in the sets. The compendium provides a valuable resource for future fundamental and applied research with C. glutamicum and contributes to a systemic understanding of this microbial cell factory. Measurement(s) Gene Expression Analysis Technology Type(s) Two Color Microarray Factor Type(s) WT condition A vs. WT condition B • Plasmid-based gene overexpression in parental strain vs. parental strain with empty vector control • Deletion mutant vs. parental strain Sample Characteristic - Organism Corynebacterium glutamicum Sample Characteristic - Environment laboratory environment Sample Characteristic - Location Germany