A pepper genetic map was constructed from F2 mapping population of 93 individuals from a cross between Capsicum annuum 'F68' and C. chinense 'Habanero'. Survey was made for the map distribution and polymorphism level of these marker groups; reverse random amplification microsatellite polymorphism (rRAMP), WRKY and amplified fragment length polymorphisms (AFLP). A total of 912 molecular markers [356 rRAMP, 190 WRKY, 305 AFLP, and 61 simple sequence repeats (SSR)] were developed in this study. The rRAMP and WRKY markers were more evenly scattered in the pepper genome than the AFLP and SSR markers, and filled the gaps not populated by the other markers. The interspecific pepper map contained 28 linkage groups with 625 linked markers and covered 3377.2 cM with an average interval of 5.9 cM. On the basis of the map, the fruit length quantitative trait loci (QTL) was analyzed and these QTL regions were detected near rRAMP and WRKY markers on the chromosome 3, 5, 11, and LG3. These marker system, map information, and detected QTLs could be one of basic information for pepper research.Additional key words: Capsicum, genetic marker, map-assisted breeding, SSR Hort. Environ. Biotechnol. 52(6):602-613. 2011.
Large amount of soybean sprouts has been produced for human consumption in Korea for a long time. Various techniques and production methods have been implied to enhance the quality and yield of soybean sprouts. The objective of the present study was to investigate the effect of persimmon fruit powder treatment on yield and nutritional value of soybean sprouts. The sprout yield was increased by 16% with the addition of 5% (w/v) persimmon fruit powder during seed soaking. Vitamin C and polyphenol content were significantly (p<0.05) increased. Similarly, essential as well as total amino acid content were also enhanced. However, the moisture content and DPPH radical scavenging potential of soybean sprout were not significantly affected. On the other hand, the mineral elements especially potassium and calcium content were reduced with the application of persimmon fruit powder. The results of the present study suggest that addition of persimmon fruit powder could improve the yield, vitamin C and amino acid content, and antioxidant potential of soybean sprouts.
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