Natural killer (NK) cells can be cultured in interleukin-2 (IL-2)- containing medium from selected human bone marrow (BM) cells obtained after the elimination of mature T and NK cells. To isolate and characterize IL-2-responsive NK progenitors in the selected BM cells, we investigated the expression of IL-2 receptors (IL-2R) on these cells. Neither CD25 (IL-2R alpha) nor IL-2R beta antigen was observed on the selected BM cells before culture. However, CD25+ cells without detectable levels of IL-2R beta antigen appeared 24 hours after culture in IL-2-containing medium. Cells were sorted from each fraction of the selected BM cells 24 hours after culture after staining with anti-CD33, anti-CD34, and anti-CD25 monoclonal antibodies. The generation of NK cells (CD3- CD56+ cells) and NK activity were observed only from the CD33-/CD34-/CD25+ cell fraction after culture in IL-2-containing medium. The frequency of IL-2-responsive NK progenitors relative to the fraction was 1/231 (95% confidence range, 1/156 to 1/289), which corresponded to the frequency relative to the total number of selected BM cells when the frequency relative to the CD33-/CD34-/CD25+ cell- fraction was converted according to the percentage of these cells in the total number of selected BM cells. These results indicated that IL- 2-responsive NK progenitors were enriched in the CD33-/CD34-/CD25+ cell fraction.

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A [Co(N)2(O)2(S)2]-type complex containing two coordinated thioether groups, trans(O)-[Co(edtda)(en)]+ (edtda = ethylenedithiodiacetate; en = ethylenediamine), has been prepared which was spontaneously resolved. The photodecarboxylation reaction of the trans(O)-[Co(edtda)(en)]+ provides trans(C,O)-[Co(CH2S(CH2)2SCH2COO)(en)]+, which has a three-membered ring. The crystal structures, UV/vis absorption, 1H NMR spectra, and chiral inversion reaction of these complexes are discussed in connection with their stereochemistry.