The present study was aimed to detect the occurrence of Marek’s disease in a native and cross-bred chickens based on gross and microscopic lesions. Seven different native as well as crossbred strains of poultry flocks were evaluated the mortality pattern based on farm as well as autopsy records and for the gross and microscopic examination of the birds suspected for Marek’s disease. Visceral lymphomas like lesions were recorded in liver, spleen, proventriculus, heart, kidney and gonads. Fragments of the affected organs were collected and placed in 10% formalin for routine histopathology. Under microscopic examination, pleomorphic population of lymphocytes and lymphoblasts were observed. The incidence of MD observed was from 12 to 61 weeks of age with peak mortality from 12 to 31 weeks of age during summer in different strains. The strain wise mortality due to MD ranged between 13.42 (random bred controls) to 36.57% (IWN×Native, F1) with an average of 26.80 %, and it was higher in female than male birds.