ObjectiveTo prospectively investigate the injury profile and the incidence rate per 1000 hours exposure during training and actual league matches in the Nigerian Women’s Premier League (NWPL) and to develop an adequate information pool, using the UEFA injury study model in order to develop appropriate injury prevention strategies.Methods241 women footballers from the eight football clubs that participated in the 2015/2016 Nigerian Women Premier league (NWPL) season were selected for the study and prospectively followed for a period of 6 months. The UEFA injury report forms and Competitive Aggressiveness and Anger Scale were sent to the various clubs, and the forms administered on them as at when due. The forms were analysed using descriptive statistics.ResultsThere was a high incidence rate per 1000 hours of exposure during training sessions (10.98 injuries/1000 hours) and matches (55.56 injuries/1000 hours); the predominant injury type was muscle rupture/strain injuries (35.49%), while moderate severity injuries were the most frequent. The predominant injury mechanism was traumatic injuries caused by contact with other players as a result of a tackle by other players (14.5%). No statistical association was established between the level of aggression and the prevalent types of injury (p=0.63).ConclusionsThe organisers of the league and indeed the referees should ensure that the rules of the game are upheld, and foul or overly aggressive play is penalised. Medical staff and coaches should consider evidence-based injury prevention strategies to reduce the risk of the common injuries sustained in the NWPL.