BACKGROUND & OBJECTIVES:The aim of the study was to evaluate the immediate efficacy of diode laser versus desensitizing paste containing 8% arginine and calcium carbonate in the treatment of dentine hypersensitivity. METHODOLOGY: 50 patients with complain of tooth hypersensitivity were randomly selected for an 8 week clinical trial with the set inclusion and exclusion criteria. Each patient was assigned to one of the two study groups: Group 1 (n=25) -8% arginine and calcium carbonate paste (Pro-Relief paste, Colgate-Palmolive Ltd, India) and Group 2 (n=25) -Diode laser(AMD, Picasso Diode, Indianapolis, Indiana), who received a baseline evaluation of tactile hypersensitivity with the help of dental explorer and an air blast hypersensitivity. The effectiveness of the therapy was assessed by VAS Scale of 10, along with the hard and soft tissue evaluation, at 4 examination periods: 1) immediately after the application of the diode laser 2) after 15 days 3) after 4 weeks & 4) after 8 weeks. RESULTS: 8% arginine and calcium carbonate showed significant results (67.27%) than diode laser (56.55%) at immediate and 4 weeks evaluation by mechanical stimulus and immediate evaluation by air stimulus. Diode laser showed highly significant results in progressive decrease in the dentin hypersensitivity till 8 weeks whereas 8% arginine showed highly significant results till 4 weeks. CONCLUSION: The immediate efficacy of 8% arginine and calcium carbonate (Colgate Pro Relief) was higher as compared to diode laser. Diode laser showed progressive reduction till 8 weeks whereas Colgate Pro Relief showed progressive reduction only till 4 weeks.