The aim of the research is to study the influence of personality traits and the life path on the rate of biological aging of adults, depending on their gender and age. Methods: diagnostic (questioning the life path, self-esteem of personality, determination of biological age) and statistical (automatic neural networks). Subjects: 987 people (including 575 women) aged 35-70 years from various regions of the Russian Federation. Continuous target variable: relative biological aging of the organism (Biological Age – Proper Biological Age). Continuous input variables: indicators of life path and personality self-esteem. Results: the general factors influencing the rate of biological aging were identified: negative factors that enhance aging - addictions and overwork; and positive factors that slow down aging are having children and professionalization. Personal indicators that changed their sign after a person retired were also found. Women changed their sign from negative to positive for indicators “work at the present time” and “study at the present time”, for men – “a positive attitude to pension reform” and professional activities of the types “Human-Technology”, “Human-Sign”. The negative strategies of organizing the life path leading to the acceleration of biological aging are highlighted: “forming the addictions and striving for excesses” and “multiplication of the load”. Positive strategies are also highlighted: “positive activity strategy” (for women and men of retirement age) and “family strategy” (for men of pre-retirement age).