Soil and water get polluted with hexahydro-1,3,5-trinitro-1,3,5-triazine (RDX) during its manufacturing, storage and use for civil and military purposes. RDX has toxic effects on living and non-living environment and is a recalcitrant compound. Therefore, the remediation of this compound is necessary. Microbial degradation of RDX can be a suitable and sustainable option to reduce its deleterious impact on the environment. Therefore, the optimization for degradation of energetic munition compound RDX employing the consortium of native bacterial species, isolated from an actual contaminated site, was performed. The experiment was planned with three independent variables (initial RDX concentration, inoculum size of microbes, and duration of the experiment) and three dependent variables (percentage removal of RDX, optical density, and nitrite release). Both independent and dependent variables were analyzed by the response surface methodology (RSM) using the Box–Behnken design. The statistical analysis using analysis of variance (ANOVA) depicted a high regression coefficient, R2 = 0.9881 with the statistically significant p-value fitted into a quadratic regression model for percentage removal of RDX. Results showed an initial RDX concentration of 40 mg/L, inoculation size 6 mL and a time duration of 12 days was optimal for the reduction of RDX up to 80.4%.

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