Substantial amounts of platelet-activating factor (PAF) were formed when IysoPAF was mixed with aspirin (e.g. 0.04% of added IysoPAF (200 nmol) was converted to PAF when mixed with aspirin (2 pmol) for 1 h). Non-enzymatic formation of PAF from aspirin and 1ysoPAF also occurs in the aqueous solution or in organic solvents in time-dependent and dose-dependent manners. Possible meanings of the non-enzymatic formation of PAF are discussed.
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