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A bacterium growing on papaverine as sole carbon and nitrogen source was isolated by incubation of soil with papaverine. The bacterium could be identified as aNocardia strain by morphological and physiological tests. When growing on papaverine, this strain excretes metabolites into the medium. Based on the structure of the metabolites 1-9 a degradation pathway is proposed.

Nocardia mutants, capable of utilizing 10 = 3-(3,4-dimethoxybenzyl)-2-hydroxy-5,6-papaverine as a sole source of carbon and nitrodimethoxy-1-indenone; gen, were induced withAf-methyl-A^-nitro-.A/-ni-11= 4-(3,4-dimethoxybenzyl)-6,7-dimethoxytrosoguanidine and mitomycin C. The isolated isocoumarin. mutants were divided in four groups, dependingThe degrading pathway of metabolites 5 and 6 on the accumulated papaverine metabolites: was investigated. Studies of the cellwall and membrane fraction of the mutants and wild-type show 4 = 2-(2-hydroxy-4,5-dimethoxyphenyl)ethanol; two inducible, particle-bound proteins with the 5 = 3,4-dimethoxyphenylacetic acid; molecular mass of 29 000 and 60 000 Da involved 6 = 2-hydroxy-4,5-dimethoxyphenylacetic acid;in papaverine degradation. Untersuchungen des Papaverinabbaus mit Papaverin-Mutanten einer Nocardia sp.Zusammenfassung: Papaverin-Mutanten wurden 10 = 3-(3,4-Dimethoxybenzyl)-2-hydroxy-5,6-mit Af-Methyl-yV'-nitro-jV-nitrosoguanidin und dimethoxy-1 -indenon; Mitomycin C induziert. Die isolierten Mutanten 11 = 4-(3,4-Dimethoxybenzyl)-6,7-dimethoxywurden in 4 Gruppen eingeordnet. Die Mutanten isocumarin. akkumulierten die folgenden Papaverin-MetaboDer Abbauweg der Metaboliten 5 und 6 wurde liten:untersucht. Untersuchungen der Zellwand-und Membran-Fraktion von Wildtyp und Mutanten 4 = 2-(2-Hydroxy^,5-dimethoxyphenyl)ethanol; zeigten, daß induzierbare, Partikel-gebundene 5 = 3,4-Dimethoxyphenylessigsäure;Proteine mit der Molekularmasse von 29 000 und 6 = 2-Hydroxy-4,5-dimethoxyphenylessigsäure; 60000 Da am Papaverinabbau beteiligt sind.

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