Objectives: This study was aimed to reveal the differences in knee valgus angle at landing as a static indicator and wobbling movement of the knee during landing as a dynamic indicator between ACL injury and uninjured athletes. Methods: This study was case-control study. There were 6 female basketball players with ACL injuries and 38 female basketball players without them, whose knee kinematics were measured using 2-dimensional video cameras during single-leg jump landings. The task was performed from 30cm-box. Knee kinematics and wobbling of the knee which was calculated by relative frontal motion to the flexion movement were compared between knees with ACL-injured and uninjured. Results: Six athletes who had confirmed ACL injuries, did not demonstrate significantly different knee valgus angle at initial contact and maximum knee flexion during landing, compared to 38 uninjured athletes. The knee valgus angles at initial contact for injured and uninjured athletes were 12.3° and 14.8° (p = 0.15), respectively. Five of six anterior cruciate ligament injured knees presented knee wobbling during landing. Relative frontal motion at 18° knee flexion was significantly greater in athletes with ACL-injured (p = 0.02). Conclusions: 84% of ACL injury presented with the knee wobbling and the frontal knee motion was greater with low knee flexion during knee wobbling, while the knee valgus angle was not significantly different. This study suggests that knee wobbling may be a biomechanical and dynamic risk factor for ACL injury in female basketball players.