Dalam budi daya tanaman cabai, penyakit rebah kecambah merupakan faktor pembatas yang sangat penting dalam produksi. Penelitian ini bertujuan menentukan kemampuan 14 isolat Trichoderma yang diisolasi dari lahan rawa lebak di Sumatera Selatan dalam mengendalikan serangan penyakit rebah kecambah. Hasil penelitian menunjukkan bahwa isolat Trichoderma dapat menghambat perkembangan penyakit rebah kecambah yang disebabkan oleh Rhizoctonia solani. Trichoderma dapat menghambat pre-emergence damping-off, post-emergence damping-off, dan keparahan penyakit berturut-turut sebesar 51.8-100%, 80-100%, dan 51.90-96.96%. Perlakuan Trichoderma juga mampu meningkatkan tinggi dan bobot basah tanaman cabai, berturut-turut 45.45-64.37% dan 37.78-81.19%. Kata kunci: agens pengendali hayati, Rhizoctonia solani, lahan rawa lebak, pemacu pertumbuhan tanaman ABSTRACT Damping-off disease is commonly occurred in chili pepper field and always caused significant effect on chili production. This experiment was conducted to determine the ability of 14 isolates of Trichoderma isolated from lowland swampy area in South Sumatera against damping-off disease. The result showed that treatment of Trichoderma significantly inhibited damping-off disease caused by Rhizoctonia solani. Treatment of Trichoderma effectively reduced the percentage of pre-emergence dampingoff, post-emergence damping-off, and disease severity by 51.8-100%, 80-100%, and 51.90-96.96%, respectively. The treatment was also capable to increase percentage of height and fresh weight of chili pepper seedling, i.e. 45.45-64.37% and 37.78-81.19%, respectively.