Background: Adequate care has to be taken to the new born child because there is large number of mortality in the age group of less than 5. Further most prevalent group is babies within day zero to day 28 after birth. Aim and Objective: The present study was undertaken to observe the risk factor analysis for neonatal sepsis and the outcome in tertiary care neonatal nursery. Material and Methods: New born babies with standard signs and symptoms of sepsis were part of the study after obtaining consent from their parents. The study was conducted between April 2019 to June 2020. Venous blood was drawn from the newborn babies after proper consent from parents and assessed for sepsis. These newborn babies were managed as per the hospital protocol and a predesigned and standard questionnaire was used to collect the data about the risk factors. Results: The most common organism isolated from blood culture was Klebsiella (36.9%). Other organisms isolated were Acinetobacter, Pseudomonas, E.coli, Staphylococcus aureus, Proteus and Citrobacter. 1% had culture positive for Non Candida albicans.  Conclusion: The most common organism isolated from blood culture was Klebsiella (36.9%).