This paper clarifies issues on vacant homes and municipal measures to survey and cope with owner-unknown homes through the nationwide questionnaire survey and the case study on Maebashi city. The results are as follows: 1)The trend differed depending on the population size of municipalities, such as the target area of survey, and the method of grasping the information for contacting each owner. 2)76.6% of municipalities that have owner unknown homes, and 72.5% of municipalities have inheritance abandoned homes, but only 15.7% of municipalities have applied the inheritance property manager system.
Nearly half of the human genome consists of repetitive sequences such as long interspersed nuclear elements. The relationship between these repeating sequences and diseases has remained unclear. Gene trapping is a useful technique for disrupting a gene and expressing a reporter gene by using the promoter activity of the gene. The analysis of trapped genes revealed a new genome element—the chromosome‐specific clustered trap (CSCT) region. For any examined sequence within this region, an equivalent was found using the BLAT of the University of California, Santa Cruz (UCSC) Genome Browser. CSCT13 mapped to chromosome 13 and contained only three genes. To elucidate its in vivo function, the whole CSCT13 region (1.6 Mbp) was deleted using the CRISPR/Cas9 system in mouse embryonic stem cells, and subsequently, a CSCT13 knockout mouse line was established. The rate of homozygotes was significantly lower than expected according to Mendel's laws. In addition, the number of offspring obtained by mating homozygotes was significantly smaller than that obtained by crossing controls. Furthermore, CSCT13 might have an effect on meiotic homologous recombination. This study identifies a transcriptionally active CSCT with an important role in mouse development.
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