Highlights d BRCT0 and BRCT1 promote ECT2 activation during cytokinesis d BRCT2 limits ECT2 GEF activity during metaphase and anaphase d BRCT2 binding to the GEF domain and RACGAP1 facilitates a narrow RhoA zone formation d Polo-like kinase 1 (Plk1) phosphorylates BRCT0 and binds each BRCT domain
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