The hydrazine and hydrazide derivatives of benzo-γ-pyrones with fluorine substituents remain an unexplored group of chemical compounds. This preliminary study reports the synthesis, structural assessment, initial microbiological screening and biological testing of the synthesized compounds on cell lines using the XTT-assay. A series of 10 novel hydrazine and hydrazide derivatives of 3-formylchromone were synthesized and their structures determined. Structural assessment consisted of elemental analysis, IR, 1H-NMR, 13C-NMR, MS and crystallographic studies. Antimicrobial activity was tested on standard strains representing different groups of microorganisms. The tested compounds were found to inhibit microbial growth. Concentrations of 0.01–1250 µmol/L were found to influence cell proliferation, demonstrating antiproliferative and stimulation of proliferation against two cell lines: the L929 cell line (mouse fibroblast cell line) and the EA.hy926 cell line (the human umbilical vein, somatic cell hybrid).

The hydrazine derivatives of benzopyrones remain an unexplored group of chemical compounds. This preliminary study investigates the influence of A-5, CH-3 and K-2 derivatives at concentrations of 1, 10, 100 nM and 1 μM on selected biochemical factors of a melanoma cell line WM-115, with regard to their potential angiogenic properties. The studied compounds were found to influence cell proliferation, as well as total protein, bFGF and FGFR1 concentration.

It has been proven that even small concentrations of certain drugs in the environment can be toxic to plants, but also fatal for animals and humans. The only proper way to prevent this phenomenon is to minimize the adverse effects due to improper waste management of medications. In developed countries, the Ministry of Health, other authorities or academic bodies set up guidelines or recommendations for the proper management of unused and/or outdated medications in the community pharmacy setting. Unfortunately, no such regulations (in the form of standard operational procedures) exist in Poland. This lack of adequate guidelines can lead to the improper and uncontrolled circulation of harmful substances, both at the level of the community pharmacy and other places disposing of medicines, as well as in the environment. The aim of this paper is to introduce the concept of medicine disposal around the world and to suggest recommendations for the proper removal of pharmaceuticals in Poland