Five fungal antagonists were evaluated in vitro against Fusarium oxysporum, applying Dual Culture Technique (Dennis and Webster, 1971) and using PDA as basal medium all the bioagents evaluated exhibited fungistatic/fungitoxic activity against F. oxysporum and significantly inhibited mycelial growth of the test pathogen over untreated control. Of the seven antagonists tested, Trichoderma viride was found most effective and recorded least linear mycelial growth (22.00 mm) with highest mycelial growth inhibition (75.66%) of the test pathogen as compared to untreated control (90.33 mm and 00.00%). Among the different bioagents tested the maximum of 75.66 per cent inhibition was found in Trichoderma viride, followed by Trichoderma harzianum and Trichoderma koningii, and the least inhibition was recorded with the Bacterial bioagents Bacillus subtilis with 43.18 per cent.

Seven isolates of Alternaria alternata were recover from diseased leaves and morphologically characterized. Among 17 germplasms Mridula, Arakta, Kabuli, and Kabuli canoor were found were found highly susceptible with a PDI ranging between 53.33 to 66.67 per cent, nine germplasms includes KRS, P13, P23, Yarcaud local, Bhagwa, Shirin anar, Super bhagwa, Kabuli yellow, and Muscat new showed susceptible reaction, Jyoti GKVR, P16 and Muscat germplasms found to be moderately resistant reaction against isolate Aa361. KRS, Mridula, Yercaud local, Muscat and Shirin anar germplasms showed moderately resistant reaction against Aa2514 remaining were showed susceptible to highly susceptible reaction. Germplsms viz., Kabuli, Arakta, Kabuli canoor shows susceptible reaction and remaining all shows resistant to moderately resistant reaction against isolate Aa356. In response to isolate Aa2427 seven germplasms shows susceptible reaction and remaining were moderately resistant except Kasuri shows resistant reaction. In response to isolates Aa2523and Aa1726 many germplasms showed susceptible to highly susceptible reaction only few showed moderately resistant reaction. On isolate Aa1728 only Muscat germplasms showed resistant and remaining were susceptible to highly susceptipble.

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