Introduction: Stature can be determined using measurements of different body parts. Forensic anthropology uses regression equation for determining stature from body part. The aim of the present study is to assess the agreement level between actual and estimated stature using regression equation from right foot length (RFL) among both sexes in Chennai region. Material and methods: In this cross sectional study, 619 subjects of both sexes with age ranging from 18 to 59 years were studied for determination of stature. Simple regression equations were formulated from RFL. They were applied for determination of stature of an individual of both sexes separately. Then they were assessed for their agreement between actual and estimated stature. Results: The present study found a satisfactory agreement but not very good agreement between actual and estimated stature with regression equation from RFL in both sexes. According to the Bland-Altman plot, there was a bias as negative predicted higher than the actual stature values and few observations were found moving beyond lower and upper limits (95% confidence limit). Conclusion: When actual stature cannot be determined, regression equation could be the most appropriate for prediction of stature using foot length in adults.