Relationship between humans and cats has negative impact associates with zoonotic diseases. It is the reason why studies on the prevalence of gastrointestinal (GI) parasites in cats are important. Some of zoonotic GI parasites in cats are Toxocara spp, Ancylostoma sp, and Toxoplasma gondii. The current study was conducted to investigate the prevalence of GI parasites in owned and stray cats in Lumajang East Java Indonesia. One hundred and twenty fecal samples were collected from owned and stray cats on November 2018 to January 2019. The samples were examined by direct smears, sedimentation and flotation techniques. Identification of parasites was determined based on the morphology of worm eggs and protozoan cysts. The results showed that gastrointestinal parasites were found in 68.33% (82/120) examined samples, respectively, 48.33% (29/60) and 88.33% (53/60) from owned cats and stray cats. We found 7 genera of parasites, 4 genera of worm eggs and 2 genera protozoan oocyst. The egg worm were Toxocara cati (40 %), Toxocara leonina. (10.33%), Ancylostoma sp. (18.33%), Diphylobothrium sp. (3.33%) and Dipylidium caninum (1.67%). The protozoan oocyst were Isospora felis (27.5%), Isospora rivolta (13.33%) and Eimeria spp. (8.33%). Toxocara cati, Ancylostoma sp. (hookworm), Diphylobothrium sp. and Dipylidium caninum were zoonotic parasites. Rate infection in younger and older cat were no significant difference. One cat can be infected one or more parasite. To conclude, the prevalence of zoonotic GI parasites both in owned and stray cats were high. It is necessary to plan a program to control this zoonotic parasites.

Aim:The aim of this study was to detect specific immunoglobulin (Ig) that could be used to determine monoclonal antibody in conjugate-making an effort for the indirect enzyme-linked immunosorbent assay (ELISA) diagnostic kit of toxocariasis in human.Materials and Methods:The study was conducted to assess the Ig profile, based on ELISA-isotyping, in mice infected with second stage larvae eggs of Toxocara cati. The optical density values of anti-T. cati mice serum IgG subclasses were analyzed by applying ANOVA factorial.Results:The specific IgG subclass in mice infected with T. cati mice was found to be IgG2β.Conclusion:Subclass of IgG, especially IgG2β, can provide leads about the use of the monoclonal antibody in conjugate making an effort for the indirect ELISA diagnostic kit.

Duthiersia expansa (D.expansa) is a cestoda of the family Diphyllobothriidae which is often found in the gut of water lizards (Varanus salvator). D. expansa worm infection is generally chronic and shows no obvious clinical symptoms but can cause weight loss, edema, ulceration of the intestinal mucosa to death. Although there have been no reports of D. expansa worm infections in humans, all types of animals have the potential to transmit diseases to humans both directly and indirectly. The present study being the first to describe morphology of surface ultrastructure of D. expansa using scanning electron microscopy in sidoarjo, indonesia, managed to compliment and verify the taxonomic valid characteristics of D.expansa as Asian Duthiersia form. Samples are collected by making incisions in the duodenum to the ileum. Sample preparation was carried out at the Airlangga University Medical School. The scanning electron microscope (SEM) analysis was carried out at the Central Institute of Life Sciences Universitas Brawijaya. The results of scanning electron microscope examination show that fan-shaped worm scoleks, bothria in the lateral scolex, existence of posterior pores in scolecs, crasspedot-shaped strobila with uterine pore and different forms of microtriches in tegument of the skoleks and strobila

Ikan kerapu bebek (Cromileptes altivelis) merupakan ikan yang memiliki nilai ekonomis tinggi dan sangat berpotensi dikembangkan di Indonesia. Salah satu spesies yang dilaporkan menginfestasi ikan kerapu bebek adalah Pseudorhabdosynochus coioidesis. Penelitian ini bertujuan untuk mengetahui tingkat prevalensi dan intensitas cacing parasit Pseudorhabdosynochus coioidesis pada ikan kerapu bebek di perairan Lampung dan Situbondo. Pengambilan sampel ikan kerapu bebek dengan ukuran 20-35 cm sebanyak 75 ekor dari perairan Lampung dan 60 ekor ikan kerapu bebek dari perairan Situbondo. Data diolah secara deskriptif dan disajikan dalam bentuk tabel. Hasil penelitian menunjukkan bahwa terdapat infestasi cacing Pseudorhabdosynochus coioidesis pada insang ikan kerapu bebek di perairan Lampung dan Situbondo. Nilai prevalensi cacing Pseudorhabdosynochus coioidesis pada pada ikan kerapu bebek di perairan Lampung sebesar 93% dengan kategori hampir selalu dan nilai intensitas sebesar 1,48 dengan kategori rendah sedangkan pada perairan Situbondo nilai prevalensi sebesar 95% dengan kategori hampir selalu dan nilai intensitas sebesar 1,33 dengan kategori rendah.

Fasciolosis is one of the endemic parasitic diseases in Indonesia and causes significant economic losses. Fasciola gigantica is the main species found to infect livestock. This research aimed to describe the morphology of F. gigantica histologically using hematoxylin and eosin staining as well as ultrastructure morphology using scanning electron microscopy (SEM). Besides, the study was also carried out to determine the prevalence and estimation of economic losses due to fasciolosis in Berau Regency. The histological identification of flukes morphology found several organs including oral sucker (OS), ventral sucker (VS), cirrus (C), genital pore (GP), uterus (U), ovary (O), tegument (Te), testis (T), and caecum (C). In this study, ultrastructure morphology was divided into anterior and midbody region. In the anterior region, there are an oral sucker (OS) with a diameter of 304.5 µm and a ventral sucker (VS) with a diameter of 571 µm. It was also found the anterior spine with a width of 25.4 µm and a height of 22.8 µm. In the midbody, the spine has a width of ± 26.7 µm and a length of ± 38.9 µm leading posteriorly. Postmortem examination results showed that the prevalence of fasciolosis in Berau regency was 21.65% or 34 of 157 cows which were positive with an estimated loss of 228,656,475.00 IDR/16,450 USD (1 USD=13,900 IDR). The amount of loss due to fasciolosis must be a particular concern in carrying out control and prevention measures. Histology and ultrastructure morphology can become a reference in the development of anthelmintic testing that has been used so far in Indonesia.